Abstract
Objective To observe the changes of the expression of transforming growth factor-β1 (TGF-β1) in serum of patients with spinal fractures and spinal cord injury, and to explore the clinical significance of TGF-β1 expression level. Methods The clinical data of 119 patients with spinal fractures were collected. Among them, 58 patients with spinal cord injury were treated as observation group and 61 patients without spinal cord injury served as control group. Sixty-seven healthy subjects were selected as the healthy group. The patients in the control group and the healthy group were treated on the 1st, 2nd, 3rd, 7th, 14th, 28th, 42th and 56th day after injury. The levels of TGF-β1 in serum were measured by enzyme-linked immunosorbent assay (ELISA) after centrifugation. The expression of TGF-β1 in the serum of the subjects was determined by centrifugation. Callus formation time and fracture healing time, and the measured and recorded data were subjected to intergroup data comparison. Results The expression of TGF-β1 in the observation group and control group was significantly higher than that in the healthy group. The levels of TGF-β1 in the serum were observed at the 2nd, 3rd and 7th day after injury. The levels of TGF-β1 were (64.5±12.8) μg/L and (43.7±13.1) μg/L on the 7th day in the observation and the control group respectively, with the difference being statistically significant (P=0.001). The levels of TGF-β1 in the observation group were (56.2±13.5) μg/L and (33.0±9.6) μg/L at 14th and 28th day respectively, significantly higher than those in the control group (P=0.002, P=0.014, respectively). The time of callus formation was (8.9±1.5) days and the time of fracture healing was (40.7±6.3) days in the observation group, significantly shorter than those in the control group (P=0.001, P=0.000, respectively). Conclusion The expression of TGF-β1 in serum of patients with spinal fractures is significantly elevated. The expression of TGF-β1 in serum of patients with spinal fractures with spinal cord injury was higher than that in patients with sinal fractures alone. The time of callus formation was shorter in patients with spinal fractures with spinal cord injury than the simple fracture patients. The spinal cord injury can promote the expression of TGF-β1, and TGF-β1 can promote the healing of spinal fractures to some extent. Key words: Fracture; Spinal cord injury; Transforming growth factor-β1; Spinal fractures healing
Published Version
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