The expression of TLR2,4,7mRNA on peripheral blood mononuclear cells in patients with chronic cystic echinococcosisinfection

Abstract

Objective To explore the expression of TLR2,4,7 mRNA on peripheralblood mono-nuclear cells (PBMCs) in patients with chronic cystic echinococcosis(CE)infection, and the level of serum IL-10. Methods The expression level of TLR2,4,7 mRNA onperipheral blood mononuclear were tested in 42 chronic CE cases and 28 normal controls(NC) by real-time fluorescent quantitative reverse transcription polymerase chain reaction(FQ-RT-PCR) method. GAPDH was selected as the internal control. The level of serum IL-10was determined in ELISA. The subjects were determined by t test. The correlations betweenTLR2, TLR4, TLR7 and IL-10 were determined by differences of expression of TLR2, TLR4,TLR7 on PBMCs and serum IL-10 in two groups of study linear correlation test. Results Theexpressions of TLR2, TLR4,TLR7 mRNA in chronic CE group were higher than those of in NCgroup. Compared with the NC group, the expressions of TLR2, TLR4 and TLR7 mRNA increasedmore than 7.3-, 3.6-, 3.6-fold, respectively. In chronic CE group, TLR2, TLR4 and TLR7mRNA expressions were 1.0729 ±0.4006, 5.0976 ±1.6682, 0. 6481 ±0. 2574, respectively.TLR2, TLR4 and TLR7 mRNA expressions were 0. 1468 ± 0.0435, 1.4067 ±0. 3279, 0. 1804 ±0.0568 in NC group, respectively. Compared with NC group, the differences of TLR2 and TLR4mRNA expression were significant (P = 0.0287, 0. 033), while the expression of TLR7 mRNAwas not difference (P =0.0862). Moreover, in chronic CE group, the level of serum IL-10was higher than that of in NC group. In chronic CE group and NC group, the level of serumIL-10 was (17.6770±1.6298) pg/ml, (9.4898 ±0.7049) pg/ml. Compared with NC group, therewas significant difference in chronic group (P<0.01). Significant positive correlationbetween TLR2 and TLR4 was found in chronic CE group, r = 0. 1135, P =0.036. Others werenot correlations. Conclusion In the development of chronic CE, TLR2 and TLR4 participatein this progression. As the receptors of antigen of cystic echinococcus, TLR2 and TLR4 canregulate the immune response through interacting with different antigens from cysticechinococcus. Meanwhile, under the participation of TLR2, TLR4 and increased serum IL-10,they will approach to Th2 immune reaction, which play an important role in chronic CE thatcan induce immune evasion.