The expression of testosterone converting enzymes in adipose tissue of polycystic ovary syndrome rat model

Abstract

Objective To establish a polycystic ovary syndrome (PCOS) rat model and compare the expression of testosterone converting enzymes in adipose tissue of PCOS rat with that of controls.Methods 21-dayold female SD rats were divided into two groups:group A(control group):feed pellets and injected with oil; group B (model group):fed with high fat diet and injected with dehydroepiandrosterone.After 9 weeks of breeding,metabolic parameters,the morphologic changes in ovary and expression of testosterone converting enzymes in adipose tissue of two group were analyzed.Results Compared with group A,fasting blood glucose,insulin,testosterone,and homeostasis model assessment for insulin resistance in group B were increased (P＜0.05).All of rats in group B had lost the regular estrous cycle,and their ovaries showed polycystic changes.The expression of 3βHSD in subcutaneous and parametrial adipose tissue was increased and that of 17βHSD1 was decreased in group B compared with group A,and the expression of 5α-reductase 1 in parametrial adipose tissue was also decreased in group B (P＜0.05).Conclusions The established PCOS rat model shows insulin resistance and a variety of metabolic abnormalities.The expression of testosterone converting enzymes in adipose tissue of this model,maybe affected by the changed level of sex steroid. Key words: Polycystic ovary syndrome ; Rat model ; Adipose tissue ; Testosterone converting enzymes