Abstract
ObjectiveTo develop a new polycystic ovary syndrome (PCOS) rat model suitable for exercise intervention.MethodThirty six rats were randomly divided into three experimental groups: PCOS rats with high-fat diet (PF, n = 24), PCOS rats with ordinary diet (PO, n = 6), and control rats with ordinary diet (CO, n = 6). Two kinds of PCOS rat model were made by adjustment diet structure and testosterone injection for 28 days. After a successful animal model, PF model rats were randomly assigned to three groups: exercise with a continuation of high-fat diet (PF-EF, n = 6), sedentary with a continuation of high-fat diet (PF-SF, n = 6), exercise with an ordinary diet (PF-EO, n = 6). Fasting blood glucose (FBG) and insulin (FINS), estrogen (E2), progesterone (P), and testosterone (T) in serum were determined by RIA, and ovarian morphology was evaluated by Image-Pro plus 6.0.ResultsBody weight, Lee index, FINS increased significantly in PF rat model. Serum levels of E2 and T were significantly higher in PF and PO than in CO. Ovary organ index and ovarian areas were significant lower in PF than in CO. After intervention for 2 weeks, the levels of 1 h postprandial blood glucose (PBG1), 2 h postprandial blood glucose (PBG2), FINS and the serum levels of T decreased significantly in PF-EF rats and PF-EO rats. The ratio of FBG/FINS was significant higher in PF-EO rats than in PF-SF rats. Ovarian morphology showed that the numbers of preantral follicles and atretic follicles decreased significantly, and the numbers of antral follicles and corpora lutea increased significantly in the rats of PF-EF and PF-EO.ConclusionBy combination of high-fat diet and testosterone injection, the obese PCOS rat model is conformable with the lifestyle habits of fatty foods and insufficient exercise, and has metabolic and reproductive characteristics of human PCOS. This model can be applied to study exercise intervention.
Highlights
Polycystic ovary syndrome (PCOS) is a heterogeneous syndrome that is characterized by anovulation, clinical and/or biochemical signs of hyperandrogenism, and abnormal ovarian morphology [1]
Ovarian morphology showed that the numbers of preantral follicles and atretic follicles decreased significantly, and the numbers of antral follicles and corpora lutea increased significantly in the rats of PF-EF and PF-EO
By combination of high-fat diet and testosterone injection, the obese PCOS rat model is conformable with the lifestyle habits of fatty foods and insufficient exercise, and has metabolic and reproductive characteristics of human PCOS
Summary
Polycystic ovary syndrome (PCOS) is a heterogeneous syndrome that is characterized by anovulation, clinical and/or biochemical signs of hyperandrogenism, and abnormal ovarian morphology [1]. Continuous administration of letrozole, 200 mg/d, to female rats for 90day starting before puberty results in a PCOS model with reproductive and metabolic features of the syndrome [8]. Continuous exposure to dihydrotestosterone (DHT) can develop an obese PCOS rat model which has typical hormonal disorders and ovarian morphological changes [9]. The model by administration of testosterone to immature female rats seeks to incorporate both the morphological changes as well as the hormonal disorders seen in PCOS. In our previous study, swimming training for 2 weeks improved significantly body weight index, insulin resistance and the androgen level in PCOS rat model induced by testosterone injection [12]
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