The expression of PPARγin fat and planceta of gestational diabetes mellitus

Abstract

Objective Measure the expression of peroxisome proliferator-activated receptor γ（PPARγ） in the fat and planceta of gestational diabetes mellitus （ GDM ） , aim to explore the role of PPARγin pathophysiology of GDM .Methods Collect the fat and placenta tissues of GDM and normal pregnant woman .Real-time PCR and Western blot were used to measure the mRNA and protein of PPARγin the two pairs of tissues .Results Compared to the normal group , the expression of PPARγin fat was lower in GDM group , both mRNA （ P =0.0 1 4 ） and protein level （ P =0.0 2 4 ） were decreased nearly 4 0%.While in the placenta , the expression of PPARγwas higher in GDM group , the mRNA and pro-tein level （ P =0.0 0 2 ） increased 1.6-fold and 2.0-fold , respectively .Conclusion Our results sug-gested that PPARγdecreased in fat and increased in placenta of GDM cases , and PPARγmay participate in the pathophysiology of GDM . Key words: PPARγ ; GDM ; Fat ; Placenta