The expression of p16,p53 induced by metallic biliary stent in canine

Abstract

Objective To explore the value of the metallic biliary stents in inducing the expression of anti-oncogenes.Methods The biliary stents were implanted in the inferior segments of CBD after a percutaneous transhepatic puncture at cholecyst in 24 dogs.After a generally observing time of half a year,the median abdominal incisions were made on the dogs under general anesthesia and the bile duet wall covered by the stents(group Ⅰ) and uncovered(group Ⅱ) were cut respectively.The expression of p16、p53、p15、Bcl-2 and K-ras in bile duct wall were detected by RT-PCR,and the positive rates of these gene expression were calculated.The differences between the two groups were analyzed by chi-spuare test for the statistics.Results The animal models were successfully set in 20 of 24 dogs.The positive rates of p16,p53 gene expression were 80% (16/20),45% (9/20) in the bile duct wall covered by the stents and 15% (3/20),0% in the bile duet wall uncovered respectively,there were significant differences between the two groups(X~2=16.94,9.17 ,P＜0.05).The positive rates of p15,Bcl-2 and K-ras gene expression were 60% (12/20),100% (20/20),0% in the bile duct wail covered by the stents and 70% (14/20),100% (20/20),0% in the bile duct wall uncovered,there were no significant differences between the two groups (P＞0.05).Conclusion The enhancement of p16,p53 gene expression have some correlations with the implantation of metallic biliary stents.The biliary stenting is strongly recommended for the patients with malignant obstructive jaundice when no contraindications exist. Key words: Gene amplification; Bile ducts; Stent; Animal experimentation