The expression of microRNA-532-5p in glioma and mechanism research

Abstract

Objective To explore the expression of microRNA(miRNA, miR)-532-5p in primary glioma and to detect the effects of miR-532-5p overexpression on proliferation, migration and invasion in U251 glioma cell line. Methods Real-time quantitative reverse transcriptase-polymerase chain reaction (RT-qPCR) was conducted to measure the expression of miR-532-5p in 30 cases of glioma tissues and traumatic brain specimens. We then analyzed the relationship of the level of miR-532-5p and the clinical pathological data of glioma patients. After transfection with miR-532-5p, the effects of on proliferation, migration and invasion were detected using methyl thiazol tetrazolium (MTT) assay, wound healing assay and transwell assay. Results The relative expression of miR-532-5p was significantly lower in glioma tissues(0.381±0.310) than in normal brain tissues (1.040±0.470, P<0.01). The level of miR-532-5p was significantly associated with Karnofsky (KPS) scores and seizures. Expression of miR-532-5p in U251 cell line (0.280±0.080) were significantly higher than those in Normal Human Astrocyte (NHA) cell line (1.021±0.069, P<0.01). The proliferation [(59.5±9.5)%, P<0.01], migration [(55.4±4.2)%, P<0.05] an invasion [(45.7±6.3)%, P<0.01] of U251 cell with miR-532-5p mimic transfection were significantly lower than those in miR-532-5p negative control after transfection. Conclusion The expression of miR-532-5p were decreased in patients with glioma. The low level of miR-532-5p was significantly associated with the KPS scores and seizures. It was shown that miR-532-5p was associated with proliferation, migration and invasion in U251 cell line and high expression of miR-532-5p was significantly associated with the KPS scores and seizures Overexpression of miR-532-5p could inhibit the capability of proliferation, migration and invasion. Key words: MicroRNA-532-5p; Glioma; Migration; Invasion; Pathological parameters