Abstract
BackgroundOsteosarcoma was locally aggressive and frequently metastasizes to the lung. However, the etiology of osteosarcoma was unknown. Thus, exploring the mechanisms behind the occurrence of osteosarcoma was important for its prediction and prevention. To investigate the usefulness of mammalian Eps15 homology domain 1 (EHD1) as a prognostic marker for osteosarcoma, the expression of EHD1 in 57 osteosarcoma patients was measured using immunohistochemistry techniques and correlated with the clinicopathological features of patients.MethodsCorrelations of EHD1 expression levels with clinicopathological features of patients were assessed using the Pearson χ2 test for categorical variables and the Student t test for continuous variables. Cumulative disease-free survival (DFS) curves and overall survival (OS) curves were plotted using the Kaplan–Meier method, and the relationship between each of the variables and survival was assessed by log-rank tests using univariate analysis. Subsequently, the parameters were tested using the multivariate Cox proportional hazards model, which was used to identify independent variables for predicting survival. EHD1 expression [P = 0.020; HR, 5.582; 95% confidence intervals (CI), 1.314–23.72] was an independent prognostic indicator of DFS in osteosarcoma patients; tumor size and EHD1 expression of osteosarcomas were independent prognostic indicators of OS in osteosarcoma patients.ResultsEHD1 protein expression was a positive expression in examined tumor tissues. The median OS time of patients with high expression of EHD1 was 46.8 months (95% CI, 29.8–63.8 months), and the median OS time of patients with low expression of EHD1 was 58.8 months (95% CI, 31.6–86.0 months). The prognosis for patients with low expression of EHD1 in osteosarcomas was significantly better than that for patients with high expression of EHD1 (log-rank test, P = 0.019).ConclusionThe expression of EHD1 was negatively correlated with DFS and OS of osteosarcoma patients; therefore, the expression of EHD1 is a prognostic marker for prediction and prevention of osteosarcomas.
Highlights
Osteosarcoma, a malignant tumor that frequently occurs in the distal femur, the proximal tibia, and the proximal humerus [1], is locally aggressive and frequently metastasizes to the lung [2]
The present study aimed to investigate the usefulness of Eps15 homology domain 1 (EHD1) as a prognostic marker for osteosarcoma
Multivariate Cox proportional hazards model analysis of the same set of patient data showed that EHD1 expression (P = 0.020; HR, 5.582; 95% confidence intervals (CI), 1.314–23.72) was independent of prognostic indicators for disease-free survival (DFS) in osteosarcoma patients (Table 2)
Summary
Osteosarcoma, a malignant tumor that frequently occurs in the distal femur, the proximal tibia, and the proximal humerus [1], is locally aggressive and frequently metastasizes to the lung [2]. EHD1 plays an important role in the control of various cellular events by regulating various proteins [5, 6], including the β1 integrins [7]. The studies were reported that EHD1 was the best-studied member of the four highly homologous mammalian proteins (EHD1–4) which regulated the endocytic recycling of membrane and associated cell surface receptors [9, 10]. These studies suggest that EHD1 plays a role in cancer invasion and metastasis. To investigate the usefulness of mammalian Eps homology domain 1 (EHD1) as a prognostic marker for osteosarcoma, the expression of EHD1 in 57 osteosarcoma patients was measured using immunohistochemistry techniques and correlated with the clinicopathological features of patients
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