Abstract
Objective To analyze the distribution of cholecystokinin (CCK) and prolactin (PRL) positive cells in rat' s brain following heat stress (HS) and febrile convulsion ( FC). Methods Acute heat stress model of seizure induced by warm water was developed in this study. Adjacent section immunohistochemical staining method was used to observe expression of CCK and PRL in cerebral cortex. Results (1) There were similar distributions of CCK and PRL positive cells in cerebral cortex of HS group. (2) Both HS and FC rats showed more positive neurons in cerebral cortex than those in control group (P ＜ 0. 01). There were significant more CCK positive neurons in cerebral cortex than that in HS group(P ＜0. 01) .however,no significant difference of PRL positive neurons was found in piriform cortex and entorhinal cortex between HS and FC group(P＞0.05) ,but the difference was significant in perirhinal cortex and parietal cortex. (3)Correlation and regression analysis of the data of CCK and PRL positive units demonstrated that the immunoreactive intensity of CCK and PRL had a positive linear correlation in cerebral cortex of HS group ( Y = 7. 939 +1. 36X, r = 0. 97, P ＜ 0. 01), but no correlation was found in cerebral cortex of FC group ( r = 0. 47, P ＞0.05). Conclusion (1) CCK may involve in anti-convulsant mechanisms in response to FC. (2) There may be a synergistic action of PRL and CCK in the central control of HS. Key words: Convulsion; Heat stress; Cholecystokinin; Prolactin; Rat
Published Version
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