The expression level and prognostic value of PD-L1 and PD-1 in cervical carcinoma patients.

Abstract

e14501 Background: By testing the expression of PD-L1 in tissues and expression of PD-1 in matched blood samples in the cohort of cervical cancers patients and cervical benign disease patients, investigate the correlation between the expression level and their prognostic value. Methods: 70 tissue samples of cervical squamous cell carcinoma and 70 tissue samples of cervical benign lesion were collected respectively, and 24 cases of the cervical squamous cell carcinoma had matched peripheral blood samples, compared to 30 cases with matched peripheral blood samples in benign lesions. Immunohistochemistry was used to detect the expression of PD-L1 in the tissues. Flow cytometry was used to detect the proportion of CD4+PD-1+ and CD8+PD-1+ subsets in peripheral blood. Log-rank and COX regression were employed to analyze the survival time, clinical features and the information above. Results: There was no difference among age, marriage age, times of pregnancy, age of menarche, menopause, ethnic cultural level and occupation, except for family history of cancer. The portion of CD4+PD-1+ T cells and CD8+PD-1+ T cells in peripheral blood positively correlated with the expression of PD-L1 in tissue samples of cervical squamous cell carcinoma patients (R2 = 0.734, P = 0.000; R2 = 0.66, P = 0.000), while there were less correlation in cervical benign patients (R2 = 0.138, P = 0.043; R2 = 0.174, P = 0.022). There were significantly differences in the portion of CD8+PD-1+T cells and PD-L1+ cancer cells between cervical cancer group and cervical benign disease group (P = 0.000, P = 0.002). The portion of CD8+PD-1+ T cells correlated with the status of menopause (chi square = 7.152, P = 0.012). There was no relationship among the tumor size, differentiation, depth of invasion and PD-1 expression in peripheral blood cervical cancer group or cervical benign disease group. Conclusions: The cervical squamous cancer patients had more CD4+PD-1+ T cells in peripheral blood and PD-L1+ cells in tissues, which correlated to the grading and prognosis of the tumor.