Abstract LB-057: The expression level and prognostic value of PD-L1 and PD-1 in cervical carcinoma patients

Abstract

Abstract Cervical cancer is one of the most common gynecologic malignant tumors that threaten the health of women. Recently some researches have found that radiotherapy combined with anti-PD-1/PD-L1 drugs might improve anti-tumor efficacy. But there is few report about this issue on cervical cancer field. In order to testify the possibility and feasibility in cervical cancer patients, we collected 70 tissue samples of cervical squamous cell carcinoma and 70 tissue samples of cervical benign lesion, of which 24 cases of the cervical squamous cell carcinoma had matched peripheral blood samples, compared to 30 cases with matched peripheral blood samples of benign lesions. We used immunohistochemistry to detect the expression of PD-L1 in the tissues and flow cytometry to detect the proportion of CD4+PD-1+ and CD8+PD-1+ subsets in peripheral blood. Then Log-rank and COX regression were used to analyze the relationship among survival time, expression of PD-L1 and proportion of PD-1+ T cells. The results indicated that the proportion of CD4+PD-1+ T cells and CD8+PD-1+ T cells in peripheral blood positively correlated with the expression of PD-L1 in tissue samples of cervical squamous cell carcinoma patients (R2=0.734, P=0.000; R2=0.66, P=0.000), while there were less correlation in cervical benign patients (R2=0.138, P=0.043; R2=0.174, P=0.022). There were significantly differences in the proportion of CD8+PD-1+T cells and PD-L1+ tissue cells between cervical cancer group and cervical benign disease group (P=0.000, P=0.002). With the increase of tumor stage, the percentage of CD4+PD-1+ T cells and CD8+PD-1+ T cells increased correspondingly. With the increase of tumor diameter and stage, the expression of PD-L1 in tumor tissue also increased. The patients with small proportion of CD4+PD-1+ T cells and low expression of PD-L1 seemed to have longer survival time. Multivariate analysis showed that the PFS time was affected by the expression of PD-L1(B=1.844, P=0.019). Taken together, the cervical squamous cell cancer patients had more CD4+PD-1+ T cells in peripheral blood and PD-L1+ cells in tissues, which correlated to the grading of tumor and associated with prognosis. Note: This abstract was not presented at the meeting. Citation Format: Yongxing Bao, Songan Zhang, Huerdidan Niyazi, Gulixian Tuluwengjiang, Lei Zhang, Yang Zhang, Weipeng Su. The expression level and prognostic value of PD-L1 and PD-1 in cervical carcinoma patients [abstract]. In: Proceedings of the American Association for Cancer Research Annual Meeting 2017; 2017 Apr 1-5; Washington, DC. Philadelphia (PA): AACR; Cancer Res 2017;77(13 Suppl):Abstract nr LB-057. doi:10.1158/1538-7445.AM2017-LB-057