The expression differences of miRNA in lung tissue and mast cells of asthmatic mice

Abstract

Objective To study miRNA expression differences in ovalbumin (OVA)-induced murine asthma models of mice and mast cells stimulated by inflammatory cytokines stimulation, and to better understand asthma deve-lopment so as to provide potential target for its prevention and treatment. Methods OVA-induced murine asthma models were validated by detecting cells in bronchoalveolar lavage fluid (BALF) and histopathology.And miRNA expression differences in the lung tissues between the model group and the normal control group were detected by real-time polymerose chain reaction PCR .After tumor necrosis factor-α(TNF-α), interleukin 12 (IL-12) stimulation, miRNA expression differences in murine mast cells P815 were detected. Results The number of total cells and eosinophil cells both increased in BALF of the model group [(12.8±2.2)×107 /L vs (5.6±2.5)×107 /L, t=4.760, P<0.05; (6.6±1.9)×107 /L vs (0.8±0.8)×107 /L, t=8.068, P<0.05]. In addition, histopathology showed more inflammatory cell infiltration in the model group than that in the normal control group, indicating that the models were validated.The expression of miRNA-155 was up-regulated approximately 5.0-fold in the lung tissues of the model group (P<0.05), while miRNA-192 showed no differences compared with the controls.After TNF-α and IL-12 stimulated P815 mast cells, miRNA-192 expressions in P815 were expression in P815 was up-regulated approximately 1.9-fold and 1.7-fold after TNF-α and IL-12 stimulation, respectively(P<0.05). Conclusions It is concluded that miRNAs are differentially expressed in the presence of OVA-induced murint asthma models and mast cells stimulated by inflammatory cytokines.These differentially expressed miRNAs may regulate the function of mast cells and involved in the pathogenesis of asthma. Key words: Asthma; miRNA; Mast cell; Inflammatory cytokines