Effect of ganoderma sporeson incubation period of asthma and mast cell tryptase in bronchial asthmatic guinea pigs

Abstract

：Objective To observe theeffect of ganoderma spores on the incubation period of asthma and the mast cell tryptase(MCT) in bronchial asthmatic guinea pigs. Methods The models of asthmatic guinea pigs wereset up by intraperitoned injection of 10％ovalhumin(OVA)for sensitizing and exposure to aerosolized 1％OVA for inducingasthma. Thirty guinea pigs were divided into three groups randomly and equally: controlgroup ( A ), asthmatic group ( B), ganoderma spores group (C). Normall saline (NS) organoderma spores were intragastriced in guinea pigs for fifteen days. The incubationperiod of asthma and airway responsiveness were detected,and the total cell numbers andclassification in bronchoalveolar lavage fluid (BALF) were studied. The lung tissues werestained with Hematoxylin-Eosin to observe the pathological changes and with anti-humantryptase antibody by using immunohistochemical technique to count the mast cell tryptase.Results ① Compared with asthma group,ganoderma spores could markedly prolong the incubation period of asthma,and reduce airwayresponsiveness, and the mast cell tryptases counts in ganoderma spores group decreasedsignificantly. Mean-while,the total cell numbers and the percentage of eosinophil of BALFin ganoderma spores group were lower than those in asthma group and the inflammationresponse in lung tissue were significantly slighter. ②Compared with NS group,asthma group had more totalcells,higher percentage of eosinophil to total cells in BALF and more mast cell tryptaseexpression in airway submucosa,alveolar walls and vascular surrounding. The inflammationresponse in lung tissue of asthma group was evident. Conclusions Ganoderma spores couldprolong the incubation period of asthma and reduce airway responsiveness,obviously relieveinflammation response in airway and lung tissue,and depress mast cell activation also. Key words: Ganoderma spores; Asthmatic guinen pigs; Incubation periodof asthma; Mast cell tryptase