Abstract
BackgroundProtein tyrosine kinase 6 (PTK6) is overexpressed in many epithelial tumors and predicts poor prognosis. However, PTK6 expression status and its role in cervical squamous cell cancer are unknown. This study aimed to investigate the expression level and clinical significance of PTK6 in early-stage cervical squamous cell cancer.MethodsQuantitative reverse transcription-polymerase chain reaction (qRT-PCR) and western blotting analysis were performed to detect PTK6 mRNA and protein expression levels in 10 freshly frozen, early-stage cervical squamous cell cancer specimens and adjacent non-tumorous cervical tissues. The expression of PTK6 was detected using immunohistochemical staining in 150 formalin-fixed, paraffin-embedded, early-stage cervical squamous cell cancer sections and 10 normal cervical tissue sections.ResultsThe mRNA and protein levels of PTK6 in cancer tissues were higher than those in adjacent non-tumorous cervical tissues. Immunohistochemical analysis showed that PTK6 was not expressed in normal cervical tissues but was overexpressed in the cytoplasm of cervical squamous cell cancer cells. The level of PTK6 expression was significantly associated with tumor grade (P = 0.020). The 5-year overall survival rate of patients with high PTK6 expression was lower than that of patients with low PTK6 expression (81.3% vs. 96.2%, P = 0.008). Multivariate Cox regression analysis showed that the expression level of PTK6 in cervical squamous cell cancer was an independent prognostic factor for patient survival (hazard ratio = 5.999, 95% confidence interval 1.622–22.191, P < 0.05).ConclusionsPTK6 is overexpressed in cervical squamous cell cancer. Increased PTK6 expression is associated with reduced 5-year overall survival. PTK6 expression is an independent prognostic predictor for cervical cancer.
Highlights
Protein tyrosine kinase 6 (PTK6) is overexpressed in many epithelial tumors and predicts poor progno‐ sis
Clinical features of included patients with early‐stage cervical squamous cell cancer A total of 150 patients were selected from our cancer center database between January 2006 and December 2011
Our data indicated that both PTK6 mRNA and protein levels were elevated in cervical squamous cell cancer and the elevated cytoplasmic expression of PTK6 was associated with tumor grade and short patient survival
Summary
Protein tyrosine kinase 6 (PTK6) is overexpressed in many epithelial tumors and predicts poor progno‐ sis. PTK6 expression status and its role in cervical squamous cell cancer are unknown. This study aimed to investigate the expression level and clinical significance of PTK6 in early-stage cervical squamous cell cancer. PTK6 is overexpressed in many epithelial tumors, including breast cancer [8], non-small cell lung cancer [9], and ovarian cancer [10], and is associated with poor patient survival. The expression level of PTK6 and its clinical relevance in cervical squamous cell cancer have not been studied. We investigated the expression level and clinical significance of PTK6 in early-stage cervical squamous cell cancer
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