The Exposure Effect of Water Pipe Smoke (WPS) on the Total Count leukocyte, Mitotic Index, Micronucleus Formation and Chromosome Aberration in Albino Male Mice

Mohammad M F Al-Halbosiy,Rakad M Kh Al-Jumaily,Fadhel M Lafta

doi:10.24126/jobrc.2015.9.2.441

Abstract

The present study was carried out to evaluate the hematological and cytogenetic effects of waterpipe smoke (WPS) in albino male mice. The investigated parameters were total count ofleukocytes (TCL), mitotic index (MI), micronucleus (MN) formation and chromosomalaberrations. The results of our study showed that, mice were exposed to WPS using a specialinhalation glass chamber (whole body exposure). Mice exposed daily to 100 puffs of WPS on thebases of (1h exposure per day). The exposure experiment continued daily for the periods of 3, 5, 7weeks consequently. The results revealed that the TCL significantly decreased in the second andthird treatment (5930, 4120 cell/ cu. mm. blood) respectively, in comparison to control (6200 cell/cu. mm. blood), while the MI decreased in all 3 treatment after 3, 5, 7 weeks. Nevertheless, boththe MN cells and chromosomal aberration remain the same percentage as the control in the firsttreatment and then began to be higher than the control in the second and third treatment. Theresults indicated that the WPS has cytotoxic and mutagenic effects, according to the obtainedresults of MN cells and chromosomal aberration assays in the three types of treatment.

Highlights

Cu. mm. blood), while the Mitotic index (MI) decreased in all 3 treatment after 3, 5, 7 weeks
The results indicated that the Water Pipe Smoke (WPS) has cytotoxic and mutagenic effects, according to the obtained results of MN cells and chromosomal aberration assays in the three types of treatment
In the second treatment after 5 weeks both the Total count leucocyte (TCL) and Mitotic index (MI) were decreased compared to the control. The count of both micronucleus cells and chromosomal aberration were increased in the third treatments after 7 weeks Similar to the second treatment, a decrease was recorded in the TCL and Mitotic index (MI)

Summary

Introduction

Cu. mm. blood), while the MI decreased in all 3 treatment after 3, 5, 7 weeks. both the MN cells and chromosomal aberration remain the same percentage as the control in the first treatment and began to be higher than the control in the second and third treatment. The results indicated that the WPS has cytotoxic and mutagenic effects, according to the obtained results of MN cells and chromosomal aberration assays in the three types of treatment. According to studies conducted by researchers at the American university of Beirut, the Water Pipe Smoke (WPS) contains a significant quantities of the same chemicals which make cigarette smoke harmful [7].

Results

Conclusion