Abstract
Objective: To findout the existence and diversity of bacteria having bio control ability against Globodera rostochiensis from six different location of potato cultivation lands in Wonosobo and Banjarnegara Indonesia. Methods: The bacteria and cyst of G. rostochiensis were isolated and Walkey-Black titration method were used to analyze the C-organic content from each location. The bio control ability of bacterial isolates was tested against cysts and eggs of G. rostochiensis followed by PCR-RAPD with two universal primers for molecular characters differentiation. Simpson’s and Shannon’s index methods were used to measure the diversity of bacteria. Findings: 45 bacterial strains were isolated. The bio control ability test showed that all strains have bio control ability against cysts and eggs of G. rostochiensis. Moreover, the PCR-RAPD showed that 33 isolates were categorized as different species. The high and medium diversity indexes indicated that the soil parasitic bacteria against G. rostochiensis have already been evolved diversely in potato cultivation lands. Novelty: The bacterial isolates that having dominant bio control ability against the golden cyst nematode from each location would be of commercial inoculant bionematicide. Keywords: Bacteria, Biological Control, Golden Cyst Nematode, G. rostochiensis, Potato
Highlights
Golden cyst nematode (GCN), Globodera rostochiensis (Wollenweber) is a plant parasitic nematode that caused a significant economic loss to the potato production
The potato plants grown in soil having high C-organic content may support the growth of G. rostochiensis better than those in the lower ones
Root diffusates that are released by potato plants which contain sugars, amino acids, protein, and other organic acids, could increase the population of cyst G. rostochiensis with stimulating 60–80% of the hatching of cyst G. rostochiensis[1,16]
Summary
Golden cyst nematode (GCN), Globodera rostochiensis (Wollenweber) is a plant parasitic nematode that caused a significant economic loss to the potato production. The egg is protected by the cyst from chemical and biological stresses found in soil and can remains dormant for more than 20 years in the absence of host plants. Since it was reported to cause economic losses for the first time in 2003 in Batu, East Java[3], the number of the infested potato cultivations has increased continuously, as well as their population. According to the previous report[5], the soil bacteria that can reduce the population of G. rostochiensis cysts and eggs are parasitic bacteria. The continuous and abundant availability of G. rostochiensis cysts and eggs may increase the number and species of their natural enemies including parasitic bacteria. The objective of this study was to elucidate the existence and diversity of parasitic bacteria against G. rostochiensis
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