The experimental research of hypoxia-inducible factor-1α small interfering RNA on the growth of human bladder cancer xenografts in mice

Abstract

Objective To observe the inhibitory effect of hypoxia inducible factor -1α(HIF-1α) siRNA on the growth of human bladder cancer xenografts in mice, and to explore the effectiveness of HIF-1α as a target for bladder cancer gene therapy. Methods pGC-siHIF-1α of stably transfected into human bladder cancer cell line T24 was inoculated to mice bladder, and the mouse model of bladder cancer was established .The tumor growth was observed by CT, and the level of tumor cells HIF-1α and CD34 was detected by immunohistochemistry (SABC), and the expression of tumor microvessel density (MVD) was evaluated according to the expression of CD34. The apoptosis index of tumor cells was determined by terminal transferase labeling (TUNEL). Results Comparison between experimental group and control group, compose got the lower rate experiment, slow tumor growth (P<0.05). HIF-1α levels decreased (P<0.01), tumor microvessel density decreased (P<0.01), and the apoptosis index was significantly increased (P<0.01). Compared with the control group, the differences between the two groups were statistically significant. Conclusions As the target of HIF-1α, the siRNA have inhibited HIF-1αexpression, contain T24 bladder cancer cells in vivo. HIF-1α may become a potential target for clinical gene therapy for bladder cancer, and provide experimental basis for anti-angiogenesis therapy of bladder cancer. Key words: Urinary Bladder Neoplasms; RNA, Small Interfering; Hypoxia-Inducible Factor 1, alpha Subunit; Mice