Abstract
Background: The exopolysaccharides (EPS) produced by yeast exhibit physico-chemical and rheological properties, which are useful in the production of food and in the cosmetic and pharmaceutical industries as well. The effect was investigated of selected carbon sources on the biosynthesis of EPS by Candida famata and Candida guilliermondii strains originally isolated from kefirs. Results: The biomass yields were dependent on carbon source (sucrose, maltose, lactose, glycerol, sorbitol) and ranged from 4.13 to 7.15 g/L. The highest biomass yield was reported for C. guilliermondii after cultivation on maltose. The maximum specific productivity of EPS during cultivation on maltose was 0.505 and 0.321 for C. guilliermondii and C. famata , respectively. The highest EPS yield was found for C. guilliermondii strain. The EPS produced under these conditions contained 65.4% and 61.5% carbohydrates, respectively. The specific growth rate ( μ ) of C. famata in medium containing EPS as a sole carbon source was 0.0068 h -1 and 0.0138 h -1 for C. guilliermondii strain. Conclusions: The most preferred carbon source in the synthesis of EPS for both Candida strains was maltose, wherein C. guilliermondii strain showed the higher yield of EPS biosynthesis. The carbon source affected the chemical composition of the resulting EPS and the contribution of carbohydrate in the precipitated preparation of polymers was higher during supplementation of maltose as compared to sucrose. It was also found that the EPS can be a source of carbon for the producing strains.
Highlights
Extracellular polysaccharides (EPS) are produced by yeast species from the Candida, Cryptococcus, Lipomyces, Pichia, Rhodotorula, and Sporobolomyces genera [1,2,3,4]
The objective of the study was to investigate the influence of carbon sources on exopolysaccharide biosynthesis by C. famata and C. guilliermondii strains isolated from kefirs
The highest EPS yield yields of exopolysaccharide (Yp/s) from 1 g of maltose was found for the C. guilliermondii strain
Summary
Extracellular polysaccharides (EPS) are produced by yeast species from the Candida, Cryptococcus, Lipomyces, Pichia, Rhodotorula, and Sporobolomyces genera [1,2,3,4] Some of these polysaccharides exhibit specific physico-chemical and rheological properties, which are useful in the production of food and in the cosmetic and pharmaceutical industries as well [5]. EPS are renewable raw materials and satisfy the environmental protection standards for classification as bioproducts For their industrial production, efficient producer yeast strains, knowledge of the regulation of biosynthetic pathways, optimization of cultivation conditions and efficient methods for purifying the product are necessary. The carbon source affected the chemical composition of the resulting EPS and the contribution of carbohydrate in the precipitated preparation of polymers was higher during supplementation of maltose as compared to sucrose. It was found that the EPS can be a source of carbon for the producing strains
Sign-in/Register to view highlights & summary Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
