Abstract
This study is focused on investigating the variations in gene expression of Bacillus subtilis 168 following acid adaptation evolution. Through simulating prolonged cultivation in a natural acidic setting, we have observed the occurrence of adaptive evolution within the strain and conducted an extensive analysis of gene expression in the evolved strain using high-throughput transcriptomics technology. The findings from this research shed light on the fundamental molecular mechanisms employed by Bacillus subtilis to adapt to acidic environments, thereby providing crucial scientific evidence for comprehending its survival strategies and potential applications in acidic settings.
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