Abstract
We present an accurate and low-cost method for the evaluation of concentration of ultrasound contrast agents, microbubbles (MBs) by utilizing light-scattering properties of MBs. Illuminated by near-infrared light, MBs act as Mie scattering particles. We used absorption spectroscopy to detect the optical density immediately after the preparation of Sonovue MBs, and after MB insonification for total of 187 MB samples. Strong linear correlation (r = 0.97) was determined between optical density of MB suspension, evaluated at 790 nm, and MB concentration in vitro (measured by hematocytometer, up to 3.3 × 107 MBs/ml).Optical density of MB suspension was assessed by simultaneously performing diffuse reflectance spectroscopy and absorption spectroscopy measurements in vitro. Further we aimed to assess MB optical density in tibialis cranialis muscles of C57Bl/CBA mice. The results of diagnostic B-scan imaging and diffuse reflectance spectroscopy shows that optical techniques are excellent for monitoring the changes in MB concentration both in vitro and in vivo.
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