The estimation of the treatment effectiveness of the experimental soft tissues septic wound with the application of polymer antimicrobial compound in the depo-form of decametoxinum

Abstract

Today septic wound treatment demands from a surgeon a multipurpose approach. For the reason of the microorganisms’ resistance to antibiotics, specialists have to use more often various dosage forms of antiseptics. According to the topical cement antibiotic form, which is used in traumatology and orthopedics, the new antimicrobial polymer compound was designed, that contains decametoxinum. Previous studies in vitro proved the sustained-release of the agent. The purpose of our experiment is to study the treatment effectiveness of the new polymer compound dosage form of the decametoxinum in comparison with other treatment methods of septic-necrotic processes of soft tissues. We estimated on laboratory animals the effectiveness of alternative treatment approaches of the septic wound management, which were caused by S.аureus and P.аeruginosa, in different groups, including those where the new antiseptics’ dosage form was used. Three groups of the septic experimental wounds were treated by the principles of monotherapy — with liquid solution of decametoxinum, depo-form of decametoxinum and with intramuscular (IM) injection of etiological antibiotic — amikacin. The animals in fourth group received multipurpose treatment — depo-form of the decametoxinum topically with amikacin IM. Fifth group — was the control one. Visual estimation, bacteriological investigations were held according to the stages of the wound healing process. Macroscopic assessment of the wounds showed the best treatment effect in groups with depo-form application, especially during the first stage of the healing process. Finally, it was revealed that in groups where different dosage forms of decametoxinum were used the effectiveness of decontamination process was almost the same. But the wounds’ redressing with depo-form of decametoxinum was carried out once in three – four days, in comparison with daily redressings of the wounds in the group where it’s liquid form was used. The sanitation effect was more vital against P.aeruginosa, then in relation to S.aureus. The best results were received in the multipurpose treatment group — the complete bactericidal effect against all microbe populations was achieved. That grants us an idea about possible potentiation of the system antibiotic by topical antiseptic.