The establishment of metabolic syndrome model by induction of fructose drinking water in male Wistar rats.

Norshalizah Mamikutty,Zar Chi Thent,Natasya Nadia Sahruddin,Shaiful Ridzwan Sapri,Mohd Rafizul Mohd Yusof,Farihah Haji Suhaimi

doi:10.1155/2014/263897

Abstract

Background. Metabolic syndrome can be caused by modification of diet by means of consumption of high carbohydrate and high fat diet such as fructose. Aims. To develop a metabolic syndrome rat model by induction of fructose drinking water (FDW) in male Wistar rats. Methods. Eighteen male Wistar rats were fed with FDW 20% and FDW 25% for a duration of eight weeks. The physiological changes with regard to food and fluid intake, as well as calorie intake, were measured. The metabolic changes such as obesity, dyslipidaemia, hypertension, and hyperglycaemia were determined. Data was presented in mean ± SEM subjected to one-way ANOVA. Results. Male Wistar rats fed with FDW 20% for eight weeks developed significant higher obesity parameters compared to those fed with FDW 25%. There was hypertrophy of adipocytes in F20 and F25. There were also systolic hypertension, hypertriglyceridemia, and hyperglycemia in both groups. Conclusion. We conclude that the metabolic syndrome rat model is best established with the induction of FDW 20% for eight weeks. This was evident in the form of higher obesity parameter which caused the development of the metabolic syndrome.

Highlights

Metabolic syndrome encompasses cluster of risk factors for cardiovascular disease which include abdominal obesity, dyslipidemia, hypertension, and hyperglycemia [1]
This is an interesting finding because the higher fluid intake and the higher calorie intake would establish the development of metabolic syndrome rat model
Past research developed metabolic syndrome rat model by induction of high carbohydrate and high fat diet (HCHF) diet for 16 weeks, we performed a new metabolic syndrome rat model in an easier way which was cost-effective and had a shorter duration of induction which was by manipulation of drinking water even with the normal food consumption for eight weeks

Summary

Introduction

Metabolic syndrome encompasses cluster of risk factors for cardiovascular disease which include abdominal obesity, dyslipidemia, hypertension, and hyperglycemia [1]. Factors that contribute to the development of metabolic syndrome are high carbohydrate and high fat diet as well as sedentary lifestyles. Metabolic syndrome can be caused by modification of diet by means of consumption of high carbohydrate and high fat diet such as fructose. We conclude that the metabolic syndrome rat model is best established with the induction of FDW 20% for eight weeks. This was evident in the form of higher obesity parameter which caused the development of the metabolic syndrome

Objectives

Methods

Discussion

Conclusion