Abstract
Objective To establish a rat model of multiple sclerosis (MS) for clinical MRI study. Methods The Lewis (LEW) rats were immunized by myelin oligodendrocyte glycoprotein peptide 35-55 (MOG35-55) emulsified with complete Freunds adjuvant/incomplete Freunds adjuvant. Twenty LEW rats were selected. Group A rats were 5, group B rats were 10 and control group rats were 5. MRI and histopathology analysis were observed on group A and control group, and clinical course were observed on group B. The clinical course was observed and the brain and spinal cord of EAE rats were scanned on 3. 0 T MR system with quadrature wrist joint coil on the third day of the acute stage of disease. The T2/T1 weighted images and Gadolinium enhanced T1 weighted images in 3D volume were obtained respectively. The magnetization transfer ratio (MTR) images were calculated with special software in workstation. Rats were sacrificed within 24 h after MRI, and the histopathological changes of central nervous system were observed. Results Twice immunization of MOG35-55 was used and a rat model of MS was successfully induced with all the rats. The model had varied clinical symptoms. The lesions of central nervous system at acute stage on group A (5 rats) were depicted on MRI. The lesions located in the brain and the spinal cord with main manifestation of hyperintensity on T2 weighed images and hypointensity on T1 weighted images. Some lesions had Gd-DTPA enhancement, and the value of MTR decreased. The infiltration of mononuclear cells around blood vessels and the extensive distribution of lesions of demyelination in brain and spinal cord were verified by histological examination. Conclusions Twice immunization of MOG35-55 could produce a rat model which mimics MS.The model is stable with higher incidence, and the antigen is cheap and obtained easily. The intravital monitoring of brain and spinal cord lesions in MOG35-55 induced rat model is possible on 3.0 T clinical whole-body MR scanner with a quadrature wrist joint coil. MOG35-55 induced rat model is an ideal MS model for clinical MRI study. Key words: Multiple sclerosis; Models; animal; Rats; Magnetic resonance imaging
Published Version
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