Abstract
DNA was isolated from gastrulae, neurulae, tailbuds and larvae of developing frog embryos by a method which reduced the level of protein to less than 1 per cent. In the presence of Micrococcus lysodeikticus RNA polymerase and the four ribonucleoside triphosphates, equal amounts of these DNA preparations primed for similar levels of RNA synthesis. The priming activity for RNA synthesis of adult frog liver DNA was greatest when the protein level was reduced to less than 1 per cent. This liver DNA primed for significantly more RNA synthesis than did liver chromatin or nucleoprotein preparations which contained the same amount of DNA. RNase treatment of low-protein DNA did not affect priming for RNA synthesis. DNA preparations from four developmental stages of the frog embryo and adult frog liver showed similar sedimentation constants in the analytical ultracentrifuge and similar melting profiles, indicating similarity of these preparations.
Published Version
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