Abstract
Objective To investigate epidemiologic and virological analysis of the outbreak of swine-origin H1N1 in Yanqing, Beijing. Methods Nasopharyngeal swab specimen were collected and adopted by real time RT-PCR to detect the swine-origin H1N1 neucleic acid. Furthermore, full length NA coding sequence were amplified with high fidelity DNA polymerase and then cloned into pCDNAII. Sequence and phylogenetic tree analysis were conducted. Results The major pathogen of this outbreak was newly emerged 2009 H1N1. NA sequence analysis showed this outbreak was caused by the same virus strain prevalent in other regions at the same period. It was shown that catalytic and glycosylation sites were highly conserved. Conclusion This outbreak was cause by the same swine-origin H1N1 virus strain. Furthermore,highly conserved catalytic and glycosylation sites of neuraminidase provides targets for the development of antiviral drugs. Key words: Influenza A virus, human; Epidemiology; Neuraminidase; Sequence analysis
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