Abstract
Objective: This research aimed to evaluate the effect of fractionation of Merremia mammosa Lour. (Mm (Lour.)) extract on diabetic wound healing by observing collagen synthesis process and to search the most potent fraction.Methods: Wistar rats were divided into five groups (n=5), i.e., K-(negative control), K+ (positive control), K1 (ethyl acetate fraction), K2 (water fraction), and K3 (n-hexane fraction). The Mm (Lour.) was extracted with ethanol 70%, then fractionated by using three solvents which have different polarity. The rats were adapted in 7 days, then induced into diabetic by streptozotocin dose 40mg/kg body weight. The wound was made by Morton excision method. Treatment was given every two days and skin biopsy was done on day 11. Analysis of collagen density was done by photomicrograph of histopathology preparations in Masson’s trichome stained by using trinocular microscope with 400x magnification in 6 fields of view, then processed by imageJ software and analyzed by appropriate statistic tool.Result: The results of this research showed that fractionation of Mm (Lour.) extract significantly enhanced diabetic wound healing based on macroscopic (percentage of wound healing) and collagen density with p-value<0.05 when compared with negative control, especially the water fraction (p=0.000). The follow up post hoc analysis showed that there was no significant (p=0.989) or there was no meaningful difference in the group of water fraction when compared to positive control.Conclusion: Water fraction is the extract fraction of Mm (Lour.) which has the most significant influence on diabetic wound healing showed by enhancement of collagen synthesis.
Highlights
Diabetes Mellitus (DM) is a chronic metabolic disease caused by the insufficient production of insulin in the pancreas or the inability of the body to effectively use insulin produced
According to the International Diabetes Federation (IDF) [1], there were 382 million people living with DM in the world by 2013 and it is estimated that this number will increase to 592 million people by 2035
Based on fig. 1(A) and 2, there was an increase in the wound healing percentage, the largest percentage of healing occurred in K2, followed by K+(gentamicin group)
Summary
Diabetes Mellitus (DM) is a chronic metabolic disease caused by the insufficient production of insulin in the pancreas or the inability of the body to effectively use insulin produced. According to the International Diabetes Federation (IDF) [1], there were 382 million people living with DM in the world by 2013 and it is estimated that this number will increase to 592 million people by 2035. As many as 25% of DM patients suffer from diabetic wounds and 85% of them experienced amputation [2]. Healing of diabetes wounds takes longer than normal wounds due to the disruption of the entire wound healing process. Treatment of diabetic foot commonly relies on gentamicin topical antibiotics. Gentamicin causes side effects which can cause skin irritation, redness, allergies, and edema [3, 4]
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