Abstract
The extracts of Paulownia tomentosa (P. tomentosa) exhibit multiple pharmacological activities. In the present study, P. tomentosa flower polysaccharides (PTFP) were extracted by water decoction and ethanol precipitation, and the immunologic modulations of PTFP against Newcastle disease (ND) vaccine was investigated in chickens. The results showed that in a certain range of concentrations, PTFP treatment can dose-dependently enhance lymphocyte proliferation. Then, 280 14-days-old chickens were randomly divided into seven groups, and vaccinated with ND vaccine except blank control (BC) group. At the first vaccination, chickens were orally administrated with PTFP at concentration ranging from 0 to 50 mg/kg once a day for 3 successive days, and the BC group was treated with physiological saline. The lymphocyte proliferation rate, serum antibody titer, and levels of interferon-γ (IFN-γ) were respectively measured on 7, 14, 21, and 28 days after the first vaccination. The results showed that PTFP at the suitable doses could significantly promote lymphocyte proliferation, enhance serum antibody titer, and improve serum IFN-γ concentrations. Taken together, these data indicated that PTFP could improve the immune efficacy against ND vaccine in chickens, and could be as the candidate of a new-type immune adjuvant.
Highlights
Paulownia tomentosa (P. tomentosa) is an extremely fast-growing tree species with multiple uses, and is widely cultivated in saline regions [1]
These data indicate that P. tomentosa flower polysaccharides (PTFP) – in combination with concanavalin A (Con-A) – exhibited the synergistic promotion effect of chicken peripheral lymphocyte proliferation
Lymphocyte proliferation rate is a direct indicator of the state of cellular immunity [21,22], and is a good criteria to study the immunomodulatory activity of drugs [23]
Summary
Paulownia tomentosa (P. tomentosa) is an extremely fast-growing tree species with multiple uses, and is widely cultivated in saline regions [1]. The bark, leaves, and flowers of Paulownia trees have been used in traditional medicine to treat infectious and inflammatory diseases in East Asia [2]. Pharmacological studies showed that the extracts from P. tomentosa could inhibit the protein tyrosine phosphatase 1B and α-glucosidase, which are important therapy targets of obesity and diabetes treatment [3], suppress the production of IL-6 and TNF-α in LPS-stimulated RAW 264.7 macrophages [2], and treat airway inflammation [4]. Multiple bioactive constituents from P. tomentosa species, including polyphenols, flavonoids, and polysaccharides, have been reported in previous studies, and polysaccharides are considered to be one of the major active ingredients in P. tomentosa. The effects of P. tomentosa flower polysaccharides (PTFP) on animal virus infectious diseases are rarely reported
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