Abstract
The enantioselective hydrolysis of racemic naproxen methyl ester by Candida rugosa lipase (CRL) was studied in aqueous buffer solution/isooctane reaction system in the presence of supercritical CO 2. The effects pressure (75–160 bar), temperature (32–42 °C) and reaction time (0.5–12 h) on the enantiomeric excesses of the product (ee p) and the substrate (ee s), enantiomeric ratio ( E), conversion ( x) and enzyme activity were investigated in a batch reactor system. The highest enantiomeric ratio achieved at 120 bar of pressure, 37 °C of temperature and 2 h of reaction time was E = 193 with x = 41.3%, ee p = 97.9% and ee s = 68.8%. CRL remained active at least for 12 h at 37 °C and 120 bar in supercritical CO 2 medium. Furthermore, enantiomeric ratio increased with increasing reaction time and reached the value of E = 236 with ee p = 98.2%, ee s = 70.0% and x = 41.6% after 12 h of hydrolysis.
Published Version
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