The efficiency of interaction between cytokines and Auxins in Micropropagation of Chrysanthemum plant (Chrysanthemum indicum L.)

Abstract

This study was conducted in the Tissue Culture Laboratory at the College of Science / Tikrit University, with the aim of micropropagation of the Chrysanthemum plant and determining the optimal concentration of the growth regulators used, In this study, a single nodes cutting from the shoots of Chrysanthemum indicum L. was used and the growth regulator (kinetin) Kin with concentrations (0.0-4.0) mg.L−1 time alone and again with the interaction (Indol-3-butyric acid) IBA at concentrations (0.0-0.6 ) mg.L−1, The results showed that the interaction between the concentrations of Kin and IBA had a higher significant effect than the use of Kin alone, Where the average of vegetative growth of Chrysanthemum plant increased as the average number of shoots, shoot length and number of leaves amounted to 7.2 shoot.plant−1, 5.27 cm and 17.0 leaf.plant−1 when added 4 mg.L−1 of Kin with 0.6 mg.L−1 of IBA to Murashige and skoog medium (MS) after being 3.3 shoot.explant−1, 3.81 cm and 5.3 leaf.shoot−1 respectively when 4 mg L-1 of Kin has added alone to MS medium, As for the rooting experiments, in which the average MS was used with the full strength of the salts and half the strength of the salts containing different concentrations of IBA growth regulator (0.0-2.0) mg.L−1 for rooting the shoots resulting from the multiplication experiments. The MS medium half the salt strength excelled and giving the highest average of rooting percentage, average number of roots and average root length of 90%, 12.5 root.explant−1 and 3.5 cm respectively when adding 1 mg.L −1 IBA to the medium.