Abstract
Hoa Binh province is one of the best places for orchids in Vietnam. The climate and environment of Hoa Binh province are favorable for the development of orchids, especially rare indigenous ones. Dendrobium anosmum Lindl., which stands out because of the unique fragrance and colors, is one of the most popular varieties in Hoa Binh province. To meet the increasing demands of the industrial market as well as to contribute to the preservation and development of genetic resources of Dendrobium sp. in Hoa Binh province, propagating D. anosmum Lindl. is a crucial step. Plant tissue culture, which has been applied to improve reproducibility of orchids for many years, is still an effective method, especially for large-scale propagation. Studies on in vitro propagation of D. anosmum Lindl. from Hoa Binh province showed that growth regulators (BA, kinetin, α-NAA) did not have a significant effect on protocorm initiation because D. anosmum Lind. from Hoa Binh province already has a high rate of regeneration. However, MS medium + 1.0 mg/L kinetin + 0.5 mg/L α-NAA + 30 g sucrose + 8.0 g agar per liter, pH 5.7–5.8 was the optimal medium to increase shoot length. The MS medium + 1.0 g activated charcoal + 30 g sucrose + 8.0 g agar per liter, pH 5.7–5.8 was the most suitable medium for shoot growth—after 6 weeks of culture, the average shoot length was 1.09 cm, the average number of leaves was 6.13, the average number of roots was 3.17, and the average root length was 1.11 cm—about 3.3, 4.17, 3.41, and 1.67 times higher, respectively, than in the control (without activated charcoal).
Highlights
Dendrobium Swartz is known as the genus with the largest number of species in the family Orchidaceae
The experiments were conducted with plant regulators that are popularly used for orchids including BA, kinetin, and α-NAA
With regard to the effect of growth regulators on the propagation efficiency of D. anosmum Lindl. orchid protocorms, in the present study, we found growth regulators have no impact on protocorm formation rate, which is not in line with previous observations on D. chrysotoxum Lindl. [22] and Aerides ringens Fisch. [23] that the rate at which germinated seeds develop into plantlets after 8 weeks treatment with growth regulator (BA, kinetin) differs from the rate in medium without BA and kinetin
Summary
Dendrobium anosmum Lindl., an orchidaceous plant called “Lưỡng diểm hạc”, “Phi diệp”, or “Giả hạc tím” is widely distributed in Thai Nguyen, Hoa Binh, Bac Giang, Ha Noi, Ha Nam, Nghe An, and Lam Dong provinces of Vietnam [6]. It is found in India, Myanmar, Laos, Cambodia, Thailand, Malaysia, Sri Lanka, Indonesia, and the Philippines. The whole plant can be regenerated from any plant parts or cells, which means faster multiplication Another advantage is that seedlings are genetically stable bringing about a practical effect on improving the quality of the seedlings and reducing the price. D. anosmum Lindl. protocorm can be formed in an environment that has sufficient nutrition, light, and humidity
Sign-in/Register to view highlights & summary 
Published Version (
Free)
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a call