The efficiency in vitro of the micropropagation of the stone fruit crops rootstock – PK SK 1

Abstract

Micropropagation is a widely used method of plant propagation in the world, incl. rootstocks of fruit crops. Although methods are generally well developed, problems often arise in the process of work, which are mainly associated with the individual characteristics of the genotypes propagated in vitro. Therefore, for each variety and rootstock, an individual study of all stages of reproduction is necessary. In the course of this work, the influence of the chelated form of iron on the propagation rate and rhizogenesis of the rootstock for stone fruit crops PK SK 1 was assessed. At the stage of micropropagation, Fe-EDDHA (6 %, 100 mg/l) and 6-BAP (0,5 mg/l; 0,75 mg/l; 1,0 mg/l). Fe-EDTA (0,5 mg/l; 0,75 mg/l; 1,0 mg/l) was used as a control. On the medium with the addition of Fe-EDDHA and 6-BAP in the amount of 0,75 and 1,0 mg/l, 9-10 new shoots are formed from one explant, which is 11-28,6 % higher than on the medium with Fe-EDTA. Also, on the medium with Fe-EDDHA, 13–26 % less vitrified shoots are formed. At the rooting stage, the usage of Fe-EDDHA (100 mg/l) with IBA 1,0 mg/l and 2,0 mg/l in the medium makes it possible to increase the rooting of plants by 12,1 and 26,9 %, respectively. On a hormone-free medium (without IBA) containing Fe-EDDHA 100 mg/l and 200 mg/l, 43,8 and 48,5 % more plants rooted than on a hormone-free medium with Fe-EDTA.