Abstract
The article presents data on the effectiveness of using various sperm preparation protocols by colloidal centrifugation (density gradient centrifugation method) before procedures of assisted reproductive technologies, including in vitro artificial insemination. The use of the method can affect the quality of sexual gametes and the viability of embryos, increase the percentage of successful embryotransplantation and contribute to the appearance of highly productive offspring.As a result of the research, the most optimal protocol of centrifugation by the method of gradient layering with indicators of the first and second centrifugation 700g – 20 min and 100g – 10 min, respectively, was determined by morphofunctional characteristics. The average rate of motile spermatozoa was 84.1±5.1%, progressive spermatozoa – 68.7±5.1%, the rate of rectilinear translational motion was 49.69 microns/sec. Morphology and viability meet quality standards.
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