Abstract
IT HAS been demonstrated repeatedly that diets made up almost entirely of plant materials are satisfactory for egg production, but require supplements such as marine products or animal protein concentrates for good hatchability (Parkhurst, 1927; Byerly, Titus and Ellis, 1932, 1933; Gillis, Heuser and Norris, 1942; Wilgus and Zander, 1944; Cravens, Halpin and McGibbon, 1946; Whitson, Titus and Bird, 1946a, 1946b; Bethke and co-workers, 1946; and Peterson, Wiese and Lampman, 1948). The announcement by Rickes and co-workers (1948a) that a biologically active crystalline compound, tentatively named vitamin B12, had been isolated from liver concentrates was soon followed by the report of Ott et al. (1948) that crystalline vitamin B12 exerted animal protein activity for chicks. During the same month Stokstad et al. (1948) announced that concentrates of a material, synthesized by a micro-organism isolated from hen feces, had high “animal protein factor” activity for chicks. Rickes and co-workers (1948b) were .
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