The effects of variation in ambient temperature alone and in combination with ε-amino-n-caproic acid on development of embryos from wheat–rye crosses (T. turgidum var. durum cv. Jori × S. cereale)

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A study was made on the effect of various temperature regimes on the in vivo development of hybrid embryos from crosses between tetraploid wheat (T. turgidum L. var. durum cv. Jori) and an inbred rye (Secale cereale L.). A day–night temperature of 17–17 °C was found to be optimum for development of embryos as classified on the basis of their morphological features and their level of totipotency in vitro. The application of ε-amino-n-caproic acid (EACA) to the female parent (tetraploid wheat) daily for a 10-day period during embryogenesis significantly improved embryo development but not independently of temperature regime. Both temperature and EACA effects were manifested by increased growth and differentiation of the hybrid embryo; endosperm development was unaffected by treatment.