Abstract
Introduction: Panax vietnamensis Ha et Grushv. (Ngoc Linh ginseng) – a new species recently discovered in Vietnam – has received much interest due to its rich content of saponins, including those unknown. This study assessed the effects of the Ngoc Linh ginseng extract fractions on proliferation and differentiation of cultured mouse neural stem cells. Methods: Whole brains were harvested from E13.5-14 Swiss mouse fetuses. Isolated cells were floating seeded to form spheroid bodies. Neurospheres were treated with one in fractions of ethanol 200-500 mg/mL, or nbutanol 200 mg/mL, or aqueous 200-500 mg/mL for 5 days. Neural stem cells could persistently generate secondary spheres. Neurospheres strongly expressed nestin, CD24 and deriving cells could differentiate into the GFAP-positive astrocyte-like cells. Results: Ginseng fractions significantly promoted neurosphere growth rate. Particularly, 200 mg/mL ginseng ethanol fraction significantly increased the neurosphere size (28.00 +/- 3.00%, p<0.0001) not showing degeneration to the 5th day. However, n-butanol and aqueous fraction could not sustain the sphere structure. Ginseng ethanol fraction also elevated in the G2/M proportion (28.73+/-0.45%, p<0.0001), up-regulated proliferation mRNA ki67 (4.605+/-6.48 fold-change, p<0.05), cycA1 (12.61+/-4.65 fold-change, p<0.0001), cycD1 (22.47+/-8.18 fold-change, p<0.0001), cycC (9.53+/-2.63 fold-change, p<0.0001) compared with those of the n-butanol or aqueous fraction-treated neurospheres. Shorten G0/G1 phase (47.08 +/-0.16, p<0.0001), up-regulation of sox2 (71.25+/-27.24 fold-change, p<0.0001) mRNA levels indicated self-renewal effect of the ginseng ethanol fraction; however, those of n-butanol and aqueous fraction-treated neurospheres suggested an inhibiting effect on the cell proliferation. Conclusion: Panax vietnamensis extract fractions had a positive effect on the proliferation of cultured neural stem cells. The ethanol fraction at 200 mg/mL could significantly promote the growth rate while still sustained the integrity of treated spheres.
Highlights
Panax vietnamensis Ha et Grushv. (Ngoc Linh ginseng) – a new species recently discovered in Vietnam – has received much interest due to its rich content of saponins, including those unknown
We investigate the potential effects of P. vietnamensis extracts on cultured neurospheres
Spheroid bodies emerging from floating cells expressed neural stem cells markers Three days since seeding, round-shape clumps of cell were seen in the culture (Figure 1A,B) 23
Summary
Panax vietnamensis Ha et Grushv. (Ngoc Linh ginseng) – a new species recently discovered in Vietnam – has received much interest due to its rich content of saponins, including those unknown. This study assessed the effects of the Ngoc Linh ginseng extract fractions on proliferation and differentiation of cultured mouse neural stem cells. Shorten G0/G1 phase (47.08± 0.16, p
Sign-in/Register to view highlights & summary Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
