The study of effects of gene of phosphate and tension homology deleted on chromsome ten inhibitors on proliferation and differentiation of neural stem cells

Abstract

Objective To investigate the effect of gene of phosphate and tension homology deleted on chromsome ten (PTEN) inhibitor bpV (pic) on the proliferation and differentiation of neural stem cells (NSCs). Methods Stem cell suspension was prepared from the cerebral cortex of the 16 days of fetal rat. The cells were divided into2 groups: group A (control group): no treatment, group B (experimental group): bpV treatment group (culture medium containing bpV). The number of stem cell spheres was observed by immunofluorescence staining, the proliferation of NSCs was detected by cell counting kit一8 (CCK-8) test, the differentiation of NSCs was detected by immunofluorescence staining, and the expression of PTEN and the mammalian target of rapamycin (mTOR) genes was detected by reverse transcriptase-polymerase chain reaction (RT-PCR) on the fifth day. Results In the experimental group, the number of stem cells per low field (×50) in the experimental group was 60±5, which was significantly higher than that in the control group (40±3, P 0.05). The expression of PTEN in experimental NSCs was 2.13 times higher than that in control NSCs (P<0.01), while the expression of mTOR in experimental NSCs was 3.62 times higher than that in control NSCs (P<0.01). Conclusion PTEN inhibitor bpV (pic) can promote the proliferation of neural stem cells and promote the differentiation of neural stem cells into neurons to a certain extent. Key words: Gene of phosphate and tension homology deleted on chromsome ten; Tumor suppressor gene; Neural stem cells; Proliferation; Differentiation