The effects of pretreatment with lipo-PGE1 on expression of Caspase-3 of kidney after hemorrhagic shock and resuscitation in rats

Abstract

Objective To observe the effects of pretreatment in rats with prostaglandin E1 on expression of kidney caspase-3, and TNF-α mRNA and iNOS mRNA after hemorrhagic shock and resuscitation(HSR). Method Totally 32 male S-D rots were randomly divided into four groups: group A (normal group, n=8), rats didn't receive any treatment; group B(sham, n=8), rats were pretreated with normal saline and underwent all experi-mentation procedures except bleeding; group C (HSR, n=8): rats were anesthetized with intraperitoneal sodium pentobarbital(30 mg/kg) and then subjected to hemorrhagic shock followed by resuscitation; group D (lipo-PGEl +HSR, n=8): rats were pretreated with lipo-PGEl one hour before HSR. The expressions of TNF-α mRNA and iNOS mRNA was measured by Northern blotting analysis and the expression of caspase-3 at 6 hours was determined by immunohistochemistry after HSR. Data were analyzed by ANOVA (SNK-q test), and P 0.05]. HSR led to overexpression of caspase-3. The expression of kidney TNF-α mRNA and iNOS mRNA also increased in HSR group, compared with normal group [(0.651±0.028) vs. (0.030±0.003), (0.524±0.022) vs. (0.026±0.003), P<0.05] and sham group[(0.651±0.028) vs. (0.029±0.002), (0.524±0.022) vs. (0.025±0.003), P<0.05]. Pre-treatment with lipo-PGEl markedly reduced the expression of Caspase-3, which was consitent with decrease in expressions of TNF-α mRNA and iNOS mRNA 6 hours after HSR[(0.250± 0.019) vs. (0.651±0.028), (0.203±0.020) vs. (0.524±0.022), P<0.050]. Conclusions Lipo-PGEl could reduce the expressions of kidney Caspase-3 after HSR. The mechanisms might be attributed to inhibiting the expression of TNF-α mRNA and iNOS mRNA. Key words: Kidney; Hemorrhagic shock and resuscitation(HSR); Lipo-PGEl; Caspase-3