The Effects of Pre-Storage Leukoreduction on the Conservation of Bovine Whole Blood in Plastic Bags.

Brena Peleja Vinholte,Fernando José Benesi,Juliana Machado Portela,Rejane Dos Santos Sousa,Antonio Humberto Hamad Minervino,Enrico Lippi Ortolani,Raimundo Alves Barrêto Júnior,Osvaldo Gato Nunes Neto,Gilson Andrey Siqueira Pinto,Francisco Flávio Vieira Assis

doi:10.3390/biology9120444

Brena Peleja Vinholte, Fernando José Benesi + Show 8 more

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https://doi.org/10.3390/biology9120444

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Abstract

Leukoreduction (LR) is a technique that consists of reducing the number of leukocytes in whole blood or blood components that can contribute to decreasing storage lesions and the occurrence of post-transfusion complications. We propose that using a blood bag with pre-storage leukocyte filtration is sufficient for blood conservation under field conditions. Ten healthy Nelore cows were used. Whole blood was sampled from each animal and stored at 2 to 6 °C in CPD/SAG-M (citrate phosphate dextrose bag with a saline, adenine, glucose, mannitol satellite bag) triple bags (Control) and in CPD/SAG-M quadruple bags with a leukocyte filter (Filter). At baseline and after 7, 14, 21, 28, 35, and 42 days (D0, D7, D14, D21, D28, D35, and D42, respectively), complete hematological, blood gas, and biochemical evaluations were determined. The filtered bag removed 99.3% of white blood cells from cattle blood, and the entire filtration process was performed in the field. There was a reduction in the number of red blood cells (RBCs) in both groups from D14 onward, with a decrease of 19.7% and 17.1% at D42 for the Control and Filter bags, respectively. The hemoglobin (Hb) concentration had variation in both groups. Potassium, pO2, pCO2, and sO2 increased, and sodium, bicarbonate, and pH decreased during storage. The filtered bag was efficient in removing white cells from cattle whole blood and could be used under field conditions. Blood stored after LR showed differences (p < 0.05) in blood gas analysis towards a better quality of stored blood (e.g., higher pH, lower pCO2, higher sO2). Further experimental studies are required to prove that blood without white cells results in a decrease in transfusion reactions in cattle.

Highlights

The development of different preservative solutions is essential for the long-term storage of blood, especially to facilitate the use of blood transfusion in human and animal medicine.stored whole blood components undergo changes known as storage lesions [1,2].The leukocyte degradation in the storage blood results in the release of cytokines, histamine, serotonin, elastase, and acid phosphatase that contribute to hemolysis and post-transfusion complications [3,4,5].Leukoreduction (LR) consists of reducing the number of leukocytes in whole blood or blood components such as red blood cell (RBC) concentrate, platelet concentrate, or plasma
The blood bag with the leukocyte filter proved to be efficient, removing 99.3% of the white cells from cattle blood, with the entire filtration process being performed in the field
There was no difference between the Control and Filter groups for the variables of RBC, Hb, and packed cell volume (PCV) (Table 1)

Summary

Introduction

The development of different preservative solutions is essential for the long-term storage of blood, especially to facilitate the use of blood transfusion in human and animal medicine.stored whole blood components undergo changes known as storage lesions [1,2].The leukocyte degradation in the storage blood results in the release of cytokines, histamine, serotonin, elastase, and acid phosphatase that contribute to hemolysis and post-transfusion complications [3,4,5].Leukoreduction (LR) consists of reducing the number of leukocytes in whole blood or blood components such as red blood cell (RBC) concentrate, platelet concentrate, or plasma. Stored whole blood components undergo changes known as storage lesions [1,2]. The leukocyte degradation in the storage blood results in the release of cytokines, histamine, serotonin, elastase, and acid phosphatase that contribute to hemolysis and post-transfusion complications [3,4,5]. Leukoreduction (LR) consists of reducing the number of leukocytes in whole blood or blood components such as red blood cell (RBC) concentrate, platelet concentrate, or plasma. The majority of studies have been performed in dogs, in which LR resulted in a 98% leukocyte and 95.1% platelet count reduction [11]. LR eliminated the post-transfusion inflammatory response in dogs receiving stored packed red blood cells [15]

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