The Effects of Platelet Rich Plasma Incorporation Towards Swelling Profile and Gel Fraction of Synthetic Coral Scaffold

Abstract

Platelet Rich Plasma (PRP) contains of bioactive molecule which is able to incorporate to scaffold for promoting bone healing. Scaffold will absorb PRP, with the result that it may affect the structural stability, shape, and degradation process itself. Consequently, this study aim is to observe the influence of incorporated PRP to synthetic coral scaffold towards the swelling profile and gel fraction. Platelet-rich plasma was prepared by a double-spinning method. The blood sample was taken from lateral tail vein of Rattus norvegicus. The synthetic coral scaffolds were made of gelatin and calcium carbonate (CaCO3). They were divided into two groups. First is PRP incorporation group and the second is non-PRP synthetic coral scaffolds as control group. The incorporation process was done by shedding scaffold into 70µ1 of PRP for 15 minutes. Swelling observation was examined by soaking scaffold in phosphate buffer saline and incubated in 37 C degrees for 24 hours. Scaffold weight was measured in every 30 minutes to observe the profile swelling and gel fraction. Used data analyzing was Independent T test. The result showed no significant differences between two groups. However, the initial measurement graphic showed the PRP incorporated scaffold swelling profile had higher number compared to the non-incorporated one. According to this study, it can be concluded that the incorporation of PRP in synthetic coral scaffold affects its swelling profile and gel fraction.