Degradation, swelling profile, and gel fraction of synthetic coral scaffold incorporated PRP or PRF

Abstract

Platelet rich plasma (PRP) and platelet rich fibrin (PRF) are sourced from human blood and have role in bone recovery. Both of them could be incorporated into scaffold for bone regeneration. Synthetic coral scaffold is a natural mimicking of sea coral designed for bone regeneration. It should have bio degradability and bio absorbability that influence new bone formation process. The purpose of this study is to investigate the degradation, swelling profile and gel fraction of synthetic coral scaffold incorporated with PRP or PRF. Synthetic coral scaffold consists of gelatin, calcium carbonate, and sodium nitrate as dispersant agent. It is divided into 3 groups, incorporated PRP, PRF, and no-incorporation. Scaffold was soaked in phosphate buffer saline (PBS) and incubated in 37° C for 24 hours. Scaffold weight was measured in every 30 minutes to observe the swelling profile and gel fraction. Degradation profile was observed after 1, 3, 6, 24, 48, 72, and 96 hours of soaking. Acceleration of degradation was measured after soaking with HC1 1N for 1, 3, 6, 24, 48, 72, 96 hours until the scaffold ran out. The result shows significant differences among PRP, PRF, and control group. PRP incorporation has slow degradation, good swelling and higher gel fraction that presents the strengthening of scaffold structure.