Abstract
The effects of pCO2 were investigated by changing the aeration rate, the purging gas and the total pressure in a chemostat cultivatioa Under glucose supply limitation, an increase in pCO2 from 44 kPa to 195 kPa resulted in 25 % decrease in cell concentration, 8 % increase in ethanol concentration, and 50 % decrease in glycerol concentration. Under oxygen supply limitation, similar dependency of ethanol and glycerol on pCO2 was observed, however, no influence of pCO2 on the cell yield was observed. The change in ethanol yield by pCO2 appeared to be caused by the equilibrium shift of pyruvate dehydrogenase system.
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