Abstract
Tissue-cultured embryonic salamander melanophores were treated with various experimental solutions in perfusion chambers and their responses recorded photographically. In some cases, measurements were made of cellular perimeter and area from the photographic records. Hypotonic media produce cellular expansion and melanin dispersion, accompanied by an increase in perimeter and area. Since the effect of MSH is very similar to this, these results provide further evidence that the action of MSH on the melanophore involves cellular uptake of water. Hypertonic media produce cellular contraction and melanin aggregation, accompanied by decreases in perimeter and area. The finding that MSH action is reversibly inhibited in hypertonic media also supports the view that water uptake is involved in MSH action. In calcium-free medium there is a contraction of the cells. MSH action is also reversibly inhibited in the calcium-free medium, making it likely that there is a calcium requirement for MSH action at the cellular level.
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