The Effects of Oral Administration of Molybdenum Fertilizers on Immune Function of Nanjiang Brown Goat Grazing on Natural Pastures Contaminated by Mixed Heavy Metal.

Abstract

Mineral development and metal smelting seriously polluted the surrounding groundwater and soil, threatening human health through the food chain. To investigate the effects of different sources of molybdenum (Mo) fertilizers on immune function of Nanjiang brown goats grazing on natural pastures under compound pollutions, fertilizing experiment was carried out in Liangshan Yi Nationality Prefecture of the Western Sichuan Plateau, China. Eighteen square hectometers of polluted meadows were fenced and were randomly divided into three groups (3 replications/group and 2 hm2/replication). A total of 54 healthy Nanjiang brown goats with an average BW of 31.6 ± 1.5kg (1year old) were used to this 30-day test (18 goats per group). The goats from CON group, AM group, and PM group were orally supplemented with deionized water, 15mg Mo/BW·d (ammonium molybdate tetrahydrate), and 15mg Mo/BW·d (potassium molybdate), respectively. Compared to CON group, the serum Fe content of grazing animals from AM group and PM group was 10.05% and 3.45% higher (P < 0.05), and the serum Cu content of grazing animals from AM group and PM group was 69.05% and 67.86% lower, respectively (P < 0.05). Mo fertilization significantly increased the levels of blood Hb, RBC, and PCV, and the activities of serum SOD, GSH-Px, CAT, and Cp of grazing goats (P < 0.05), and also extremely decreased the MDA content of experimental goats fed Mo compared to the control goats (P < 0.05). Compared to CON group, the activities of serum IgG, IgA, IgM, IL-2, and TNF-α of grazing animals from AM group and PM group were significantly increased (P < 0.05), and the levels of serum IL-6 and IL-1β of grazing goats from AM group and PM group were extremely decreased (P < 0.05). In summary, oral Mo fertilizers can alter the contents of serum mineral elements, reduce oxidative stress, improve immune function, and relieve the toxic damage of goats grazing on contaminated natural grasslands.