Abstract
In this research, the effect of oral administration of different doses of hydroalcoholic extract of si lymarin on the status of trace elements with antioxidant pr operties (Se, Cu, Zn, Mn and Fe) was studied. Fifty male rats were randomly used in five groups includi ng healthy control group (treated by saline), diabe tic control group (treated by saline) and three diabeti c groups that were daily treated by different oral dose of silymarin (100, 175 and 250 mg kg -1 ). Diabetes was induced by intraperitoneal injectio n of streptozotocin (50 mg kg -1 ) in rats. Rats were orally treated for 14 days by gavage. Weight was measured before STZ injection, 7 and 14 days after treatment . The blood glucose was measured before injection o f STZ, 3 days after injection and 7 and 14 days after diabetes was induced (under treatment by silymarin ). At the end of the study, blood samples were taken b y heart puncture. Results showed that the average weight increased during the 14 days of treatment wi th silymarin and the blood glucose was decreased. Hydroalcoholic extract of silymarin increased trace elements levels in treated groups by dose-dependen t manner. The present study showed that in diabetic r ats, high therapeutic doses of silymarin had a majo r effect on serum antioxidant factors. Results also s howed that hydroalcoholic extract of silymarin increased the body weight and decreased serum glucose concentration at the end of the treatment period . Silymarin in a dose dependent manner could improve trace element levels in diabetic rats. Therefore, i t may cause reduction of oxidative stress in the diab etic rats.
Highlights
Silymarin is a flavonoid found in the herb milk thistle standardized extract of flavonolignans silybinin, isosilybinin, silydianin and silychristin and other minority compounds from the seeds of Silybum
Fifty male rats were randomly used in five groups including healthy control group, diabetic control group and three diabetic groups that were daily treated by different oral dose of silymarin (100, 175 and 250 mg kg−1)
The present study showed that in diabetic rats, high therapeutic doses of silymarin had a major effect on serum antioxidant factors
Summary
Silymarin is a flavonoid found in the herb milk thistle standardized extract of flavonolignans silybinin, isosilybinin, silydianin and silychristin and other minority compounds from the seeds of Silybum. Copper, zinc, manganese and iron are essential components of metalloenzymes such as Se-cys containing glutathione peroxidase, Cu/Fe cytochrome C oxidase or different types of superoxide dismutases. All of these trace elements are important in intra- and extra-cellular antioxidant defense (Flores et al, 2011). The disturbance in trace elements status and increased oxidative stress in diabetes mellitus may contribute to the insulin resistance and development of diabetic complications (Basaki et al, 2012). Basaki et al (2012) reported that copper levels in diabetic patients were lower than the control group. Effect of silymarin on kidneys of rats suffering from alloxan-induced diabetes mellitus was investigated (Soto et al, 2010). Haddad et al (2011) reported that silibinin have antioxidant and hepatoprotective effects in rats with nonalcoholic steatohepatitis
Sign-in/Register to view highlights & summary Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callMore From: American Journal of Animal and Veterinary Sciences
Disclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
