Abstract
Abstract Shephard's synthetic sea water medium for Acetabularia (Shephard, 1970) was compared with natural sea water-based media. Cells in natural sea water supplemented with nitrate, phosphate, mineral micronutrients and vitamins grew faster and formed caps sooner than those grown in the synthetic medium. This difference was not overcome by the addition of soil–water extract, other trace elements, higher levels of nitrate or phosphate, or a greater number of vitamins. On the other hand, sea water had no effect on maximum cell length, percentage of cells forming caps, the time between cap initiation and cyst formation, or the time needed for gamete release. Since three species of Acetabularia have completed their life-cycle in Shephard's medium, and two species have been maintained in it for several generations, the sea water seems to be providing a stimulatory factor rather than an essential nutrient. The variability of natural sea water and the ease of preparing large quantities of sterile synthetic med...
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
