Abstract
BackgroundTo evaluate and compare the effects of ethanolic extracts of Malaysian propolis and Brazilian red propolis at different concentrations on the migration and proliferation of fibroblast cells.MethodsMalaysian and Brazilian red propolis crude samples were extracted using ethanol. Their wound healing effects were tested in vitro on the normal human fibroblast cell line CRL-7522. Cell migration and proliferation assays were carried out using propolis concentrations of 1, 10, 100, 250, 500 and 1000 μg/mL. The data were analyzed using one-way ANOVA and post hoc Bonferroni tests (α = 0.05).ResultsMalaysian and Brazilian red propolis followed a concentration-dependent increasing and decreasing trend. Malaysian propolis showed the fastest migration rate at 250 μg/mL which was statistically significant (p < 0.05) and maximum proliferation at 500 μg/mL with no significant difference (p > 0.05) compared to control. Brazilian red propolis showed a slight increase in migration and proliferation at 10 and 100 μg/mL, respectively with no significant difference (p > 0.05) compared to control, while concentrations above these conferred inhibitory effects.ConclusionMalaysian and Brazilian red propolis show potential to assist in wound healing, depending on their concentration.
Highlights
To evaluate and compare the effects of ethanolic extracts of Malaysian propolis and Brazilian red propolis at different concentrations on the migration and proliferation of fibroblast cells
Cell migration assay Malaysian propolis at concentrations of 1, 10 and 250 μg/mL indicated visually faster wound closure compared to the control, while the other concentrations appeared visually similar to the control
The ability of cells to migrate into wounds in the presence of dimethyl sulphoxide (DMSO) was similar to control at 12 h, but at 24 and 48 h the migration rate was slower than all other groups (Fig. 1)
Summary
To evaluate and compare the effects of ethanolic extracts of Malaysian propolis and Brazilian red propolis at different concentrations on the migration and proliferation of fibroblast cells. Given the limited studies carried out on the effects of Brazilian red propolis on the proliferative phase of wound healing and the lack of scientific study on Malaysian propolis, this present study was aimed to evaluate and compare the effects of ethanolic extracts of Malaysian propolis and Brazilian red propolis at different concentrations (1, 10, 100, 250, 500 and 1000 μg/mL) on the migration and proliferation of connective tissue fibroblast cells, and to determine the optimum association curve between time and migration of the fibroblast cells at the different concentrations of Malaysian propolis and Brazilian red propolis
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