Abstract
This study aimed to investigate the mechanism of intervening type II diabetes by flavonoid compound from Agrimonia pilosa Ledeb in vitro. We found flavonoid compound exhibited moderate peroxisome proliferator-activated receptor gamma agonist activity which was lower than that of rosiglitazone by luciferase reporter assay. 3T3-L1 cell differentiation was induced typically in the presence of various concentrations of flavonoid compound or pioglitazone for assaying insulin sensitization. We found that flavonoid compound effectively promoted 3T3-L1 preadipocyte differentiation and lipid deposition, but its intensity was lower than that of pioglitazone. Quantitative polymerase chain reaction assays showed that flavonoid compound promoted the expression of peroxisome proliferator-activated receptor gamma, CCAAT enhancer binding protein-α, and sterol regulatory element-binding protein 1 in a dose-dependent manner. As a result, the expressions of Glut4 and adiponectin were significantly enhanced. Additionally, the expressions of AP2 and lipoprotein lipase induced by flavonoid compound were lower than those by pioglitazone. These findings indicate that flavonoid compound from Agrimonia pilosa Ledeb exhibits the good property of partial peroxisome proliferator-activated receptor gamma agonism, and shares similar insulin-sensitizing effects with pioglitazone, but has lower adipogenic capacity. It is implied that flavonoid compound has a promising prospect of fighting insulin resistance as a partial agonist of peroxisome proliferator-activated receptor gamma.
Published Version
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