Abstract
The pattern of chromosomal aberrations (CA) was studied by fluorescence in situ hybridization (FISH) technique (whole chromosomes #1 and #4 painting) in workers occupationally exposed to any of the four following conditions: acrylonitrile (ACN), ethyl benzene (EB), carcinogenic polycyclic aromatic hydrocarbons (c-PAHs), and irradiation in nuclear power plants (NPP), respectively. Decrease in the relative frequency of translocations was observed in EB group, and an increase in reciprocal translocations in ACN and NPP-exposed groups. An increase in a relative number of insertions was registered under all four conditions (significant at ACN, EB, c-PAHs, quasisignificant at NPP-exposed groups). Significant differences in the percentage of lymphocytes with aberrations on chromosome #1 (58.8 ± 32.7%, versus 73.8 ± 33.6% in the controls, P < 0.05), and chromosome #4 (47.0 ± 34.1%, versus 29.4 ± 32.2%, P < 0.01) were found in workers exposed to ACN. Similarly, a decrease in the proportion of cells with aberration on chromosome #1 (61.0 ± 24.0%, versus 73.8 ± 33.6%, P < 0.05) and an increase on chromosome #4 (45.6 ± 24.6%, versus 29.4 ± 32.2%, P < 0.05) were observed in workers exposed to EB. Frequency of aberrant cells (%AB.C.) as well as genomic frequency of translocations ( F G/100) increased with age ( P < 0.001). Aging also increased the percentage of translocations and reciprocal translocations ( P < 0.05), but decreased the relative number of acentric fragments ( P < 0.01). Smoking led to significantly increased F G/100 ( P < 0.05), but did not affect the pattern of chromosomal aberrations. Our results seem to indicate that different carcinogens may induce a different pattern of chromosomal aberrations.
Published Version
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