The effects of ethanol-consumption on cell proliferation in the brain of the post-hatch domestic chick

Abstract

Event Abstract Back to Event The effects of ethanol-consumption on cell proliferation in the brain of the post-hatch domestic chick Tímea Bodogán1*, Gergely Zachar1, Eszter Bálint1, Szilvia Mezey1 and Andras Csillag1 1 Department of Anatomy, Histology and Embryology, Semmelweis University, Hungary Alcohol administration causes apoptosis in most areas of the brain in rodents, however alcohol might cause increased cell proliferation in some regions. Although the domestic chick is widespread as an embryonic model of different neurotoxic effects, the postnatal effects of alcohol are less studied.In our study the formation of new cells in young chicks was observed after ethanol treatment. Three groups of chicks received 5 g/kg bodyweight, 2.5 g/kg or 0 g/kg ethanol daily for one week, administered via an oesophageal tube. On day 8 bromo-deoxi-uridine (BrdU) was injected i.p. to label newly generated cells. After 24 hours the brains were dissected and BrdU-labelled cells were detected by fluorescent immunohistochemistry. The labelled cells in the hippocampus, nucleus accumbens and medial striatum were counted using a confocal microscope. The low dose of ethanol (2.5 g/kg) decreased the number of newly generated cells compared to the control group in the accumbens and hippocampus but not in the medial striatum, however no such decrease was observed in the 5 g/kg group. The brain of chicks is likely to compensate for the stronger neurotoxic effect of the higher dose of ethanol by increasing proliferation of glial or neuronal cells, as observed in rodents. Post-hatch chicks are more developed than newborn rats, and the temporal pattern of their brain maturation is more similar to human neural development than that of rodents. Our results suggest, that chicks can be an ideal model to study the effects of alcohol on early postnatal brain development. Conference: 12th Meeting of the Hungarian Neuroscience Society, Budapest, Hungary, 22 Jan - 24 Jan, 2009. Presentation Type: Poster Presentation Topic: Pathophysiology and neurology - non-degenerative disorders Citation: Bodogán T, Zachar G, Bálint E, Mezey S and Csillag A (2009). The effects of ethanol-consumption on cell proliferation in the brain of the post-hatch domestic chick. Front. Syst. Neurosci. Conference Abstract: 12th Meeting of the Hungarian Neuroscience Society. doi: 10.3389/conf.neuro.01.2009.04.121 Copyright: The abstracts in this collection have not been subject to any Frontiers peer review or checks, and are not endorsed by Frontiers. They are made available through the Frontiers publishing platform as a service to conference organizers and presenters. The copyright in the individual abstracts is owned by the author of each abstract or his/her employer unless otherwise stated. Each abstract, as well as the collection of abstracts, are published under a Creative Commons CC-BY 4.0 (attribution) licence (https://creativecommons.org/licenses/by/4.0/) and may thus be reproduced, translated, adapted and be the subject of derivative works provided the authors and Frontiers are attributed. For Frontiers’ terms and conditions please see https://www.frontiersin.org/legal/terms-and-conditions. Received: 04 Mar 2009; Published Online: 04 Mar 2009. * Correspondence: Tímea Bodogán, Department of Anatomy, Histology and Embryology, Semmelweis University, Budapest, Hungary, timee8@freemail.hu Login Required This action requires you to be registered with Frontiers and logged in. To register or login click here. Abstract Info Abstract The Authors in Frontiers Tímea Bodogán Gergely Zachar Eszter Bálint Szilvia Mezey Andras Csillag Google Tímea Bodogán Gergely Zachar Eszter Bálint Szilvia Mezey Andras Csillag Google Scholar Tímea Bodogán Gergely Zachar Eszter Bálint Szilvia Mezey Andras Csillag PubMed Tímea Bodogán Gergely Zachar Eszter Bálint Szilvia Mezey Andras Csillag Related Article in Frontiers Google Scholar PubMed Abstract Close Back to top Javascript is disabled. Please enable Javascript in your browser settings in order to see all the content on this page.