The effects of different extraction conditions on the polyphenol, flavonoids components and antioxidant activity of Polyscias fruticosa roots

Abstract

Polyscias fruticosa is an herbal plant having a myriad of medicinal purposes, especially in antioxidant activities, anti-inflammatory and antipyretic. This study was carried out to evaluate the effects of different extraction conditions on the polyphenol, flavonoids content extraction and antioxidant activity of the Polyscias fruticosa root extract. DPPH and ABTS methods were employed to measure free radical scavenging activities. The total polyphenol, flavonoids content was calculated by gallic acid and quercetin equivalent, respectively. The absorbance is measured by using UV-Visible spectrophotometry. The results shows that the conditions in which the highest extraction yield was attained consisted of 90% ethanol, the ratio between material and ethanol of 1:20 g/mL, extraction time of 3 hours and extraction temperature of 30°C. This extract showed the highest polyphenol content (96,09 μg gallic acid equivalent/mg) and the highest flavonoid content (58,30 μg quercetin equivalent/mg of dry extract). Moreover, DPPH and ABTS antioxidants activity results showed that Polyscias fruticosa root extracts exhibited the IC50 value of 96,14 μg/mL and 38,76 μg/mL, respectively. These results indicate that Polyscias fruticosa roots can be used in dietary applications with the potential to reduce oxidative stress.